Labbench activity exercise 3: water potential of potato cores this activity is very similar to exercise 2, except that you use cores from potatoes instead of dialysis bags. Hypothesis: if potato cores are submerged into different solutions with varying water concentration, then the mass of the cores will change depending on the amount of water present materials: goggles #2 cork borer 1 potato 1 scalpel 1 cm ruler 6 plastic cups plastic wrap or foil pencil 100 ml distilled water 100 ml 02. This practicdal assessment is designed to determine the water porential of potato tissue or any suitable plant tissue, using three quick methods: change in length change in mass change in the density of the sucrose solution, with the aid of methylene blue solution. Objective the objective of this lab is to measure the water potential of potato tuber tissues we will monitor water uptake or loss by tissues in different sucrose solutions to identify an isotonic solution with water potential equal to that of the tissue.
The water potential of the potato would decrease because water moves from a high water potential region to a low potential region, and a dehydrated potato cell is. In tubers of high water potential, measurements on excised tissue were as much as 05 megapascals lower than the pressure chamber, probably due to turgor-driven expansion of the sample when freed from constraints imposed by surrounding tissue. We left the potato tissue for a few hours as we were running out of time and couldnt waste lessons but it wud b betr 2 leave it for 24 hours to let the water potentials change as much as possible to the extent that they should do. The percentage change of mass was then calculated explain how a student could use the data in table 212 to determine the water potential of the potato tuber tissue • one kb of double stranded dna has a length of 034 µm (a) the dna in the nucleus of a cell from a fruit fly (drosophila).
Understanding the osmotic potential of plant cells is a key part of understanding cellular processes here we present two methods of determining osmotic potential of plant tissues using potatoes method one, the standard protocol for measuring weight change of tissues in varying osmotic solutions. Investigate the osmosis of potato cells in various salt solutions 2525 words | 11 pages investigate the osmosis of potato cells in various salt solutions introduction i have been asked to investigate the effect of changing the concentration of a solution on the movement of water into and out of potato cells. This sucrose solution gave an osmotic potential value of -4986 x 10-6, which is an approximation of the water potential of the potato tissue figure 2 relationship between mean weight change in potato cylinder and osmotic potential of sucrose solutions. Labbench activity calculating water potential water potential is calculated using the following formula: water potential (ψ) = pressure potential (ψ p) + solute potential (ψ s) pressure potential (ψ p): in a plant cell, pressure exerted by the rigid cell wall that limits further water uptake. Measurement of the water potential of plant tissues general introduction solution in which the weight and volume of the tissue does not change, indicating neither a net loss nor a net gain in water such a situation measurement of the water potential of plant tissues -----± ~ ' :.
The change in length or weight of the potato is the dependent variable in this experiment to measure the change in weight, use a balance to measure the initial and the final weight of the potato strips. Percentage of change in mass is decreasing thusly we can conclude thatthe difference of the initial from the final and as it gets greater thatmeans the mass of the potato changed massivelyaccording to the osmosis theory as the concentration of the sucrosesolution increases the particle’s water potential increases and becomeshigher than the. Times on the leaf water potentials of cut leaf tissue measured by the dye method species: dogwood, cormis 1961, lemee and gonzalez 1965), the proper length of immersion for a particular species should be deter- in water content per unit change in water potential. From your graph what do you estimate the water potential of the potato tissue to be 4 does the increase in tissue weight and length resulting from gaining water in the less negative water potential solutions eventually plateau and reach a maximum.
Determining the water potential of potato tuber cells length: 2562 words (73 double solution outside the cells is isotonic to the molarity of the sucrose inside the cells then there will be no change in weight of the potato disks [tags: papers] - determining the concentration of the cell sap in potato storage tissue aim: to. The water potential of the potato tissue can be expressed in terms of the molarity of sucrose solution that causes no change in length of the tissue b by weight. Best answer: 1-cut the potato tissue into several strips (about 10, 2 for each dish) exactly equal in length and thickness (around 50 mm in length) using a ruler and cork borer 2- prepare 5 petri dishes 3- measure for example 50 cm3 of distilled water into each dish 4. 7 calculate the water potential of the potato cells as follows find the point on your graph corresponding to a percentage mass change of zero the molarity of sucrose corresponding to this zero mass change can now be read from the horizontal axis.
Water potential of the potato tissue explain in terms of water potential, the percentage change in length of the potato chips that occurred in water. In this exercise you will determine the water potential of potato cells at room temperature and ambient pressure by placing cores of potato tissue in sucrose solutions of different concentrations and measuring the net movement of water in each case. Potato tuber tissue is neededto investigate furtheranapparent relationship between water potential and the susceptibility of stored tubers to attack by soft rot erwinias (15. As osmosis is defined as the movement of water molecules across a partially permeable membrane from an area of high water concentration to an area of low water concentration, i predict that as the water concentration decreases in the potato pieces the overall mass and length will decrease too.